Tumor necrosis factor receptor p75 (TNF-R p75, or TNF-R2) is a 75 kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II (TNFsRII) gene localizes to 1p36 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFsRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of non-lymphoid cells. The standard product used in this kit is recombinant human TNFsRII, consisting of the 24-206 amino acid sequence with a molecular mass of 20 kDa.

ScienCell's human TNFsRII ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human TNFsRII-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human TNFsRII in the sample that is captured on the strips.

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