ScienCell's GeneQuery™ Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:
- M1 microglia phenotype markers: TNF, NOS2, IL1B
- M2 microglia phenotype markers: IL10, ARG1, MRC1
- Pericyte markers: ACTA2, PDGFRB, CSPG4
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Evaluation of neural differentiation
- Astrocyte markers: GFAP, S100B, GLUL
- Immature neuron markers: NEUROD1, DCX, TUBB3
- Mature neuron markers: MAP2, RBFOX3, SYP
- Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
- Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1
Note : all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
货号 | GK127 |
产地 | 美国 |
缩写 | GQH-BDM |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | -20°C |
运输 | 干冰 |
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