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Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.
MDF from ScienCell Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse skin. MDF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MDF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. MDF are negative for mycoplasma, bacteria, yeast, and fungi. MDF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MDF in vitro.
货号 | M2300-57 |
产地 | 北美 |
缩写 | MDF |
规格 | 5 x 10^5 cells/vial |
用途 | 科研 |
储存 | 液氮 |
运输 | 干冰 |
小鼠细胞系统
小鼠细胞系统
货号 | 产品名称 | 规格 | 价格 | 指令 | |
M4200 | 小鼠肾系膜细胞 | 5 x 10^5 cells/vial | $446 | + | |
M4200-57 | 小鼠肾系膜细胞 | 5 x 10^5 cells/vial | $470 | + | |
M5300 | 小鼠肝星状细胞 | 5 x 10^5 cells/vial | $477 | + | |
M5300-57 | 小鼠肝星状细胞 | 5 x 10^5 cells/vial | $544 | + | |
M5340 | 小鼠肝巨噬细胞 | 1 x 10^6 cells/vial | $372 | + | |
M5340-57 | 小鼠肝巨噬细胞 | 1 x 10^6 cells/vial | $417 | + | |
M5540 | 小鼠脾细胞 | 10 x 10^6 cells/vial | $198 | + | |
M5540-25 | 小鼠脾细胞 | 25 x 10^6 cells/vial | $390 | + | |
M5540-57 | 小鼠脾细胞 | 10 x 10^6 cells/vial | $238 | + | |
M5550 | 小鼠脾脏巨噬细胞 | 1 x 10^6 cells/vial | $372 | + | |
M5550-57 | 小鼠脾脏巨噬细胞 | 1 x 10^6 cells/vial | $417 | + | |
M6200 | 小鼠心肌细胞 | 1 x 10^6 cells/vial | $446 | + | |
M6200-57 | 小鼠心肌细胞 | 1 x 10^6 cells/vial | $489 | + | |
M6300 | 小鼠心脏成纤维细胞 | 5 x 10^5 cells/vial | $446 | + | |
M6300-57 | 小鼠心脏成纤维细胞 | 5 x 10^5 cells/vial | $489 | + | |
M7540 | 小鼠胚胎成纤维细胞 | 1 x 10^6 cells/vial | $143 | + | |
M7540-2-mt | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 2 x 10^6 cells/vial | $44 | + | |
M7540-2-mt-v10 | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 2 x 10^6 cells/vial. 10 vials/pk | $381 | + | |
M7540-2-mt-v20 | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 2 x 10^6 cells/vial. 20 vials/pk | $777 | + | |
M7540-2-mt-v5 | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 2 x 10^6 cells/vial. 5 vials/pk | $201 | + | |
M7540-5 | 小鼠胚胎成纤维细胞 | 5 x 10^6 cells/vial | $278 | + | |
M7540-5-mt | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 5 x 10^6 cells/vial | $102 | + | |
M7540-57 | 小鼠胚胎成纤维细胞 | 5 x 10^6 cells/vial | $327 | + | |
M7540-mt | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 1 x 10^6 cells/vial | $28 | + | |
M7570 | 小鼠胚胎成纤维细胞 | 1 x 10^6 cells/vial | $143 | + | |
M7570-5 | 小鼠胚胎成纤维细胞 | 5 x 10^6 cells/vial | $283 | + | |
M7570-5-mt | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 5 x 10^6 cells/vial | $105 | + | |
M7570-mt | 小鼠胚胎成纤维细胞--丝裂霉素处理 | 1 x 10^6 cells/vial | $25 | + |