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小鼠胚胎成纤维细胞--丝裂霉素处理
Mouse Embryonic Fibroblasts-mitomycin C treated from CD1
品牌:
ScienCell
货号:
M7540-2-mt
价格:
$44
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Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells. MEF-mt from ScienCell Research Laboratories are isolated from embryonic day 14 CD-1 mouse embryos. These cells have been treated with mitomycin C to prevent further cell division. They are cryopreserved at passage 3 and delivered frozen. Each vial contains 1 x 106 cells in 1 ml volume. MEF-mt are characterized by immunofluorescence with antibody specific to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast, and fungi. MEF-mt are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MEF-mt are not recommended for expanding as mitomycin C treatment prevents further cell proliferation. Recommended Medium It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF-mt in vitro.
货号M7540-2-mt
产地北美
缩写MEF-mt
规格2 x 10^6 cells/vial
用途科研
储存液氮
运输干冰
小鼠细胞系统
小鼠细胞系统
货号 产品名称 规格 价格 指令
M1830 小鼠脊髓星形胶质细胞 5 x 10^5 cells/vial $446 +
M1830-57 小鼠脊髓星形胶质细胞 5 x 10^5 cells/vial $489 +
M1840 小鼠脑干星形胶质细胞 5 x 10^5 cells/vial $446 +
M1850 小鼠中脑星形胶质细胞 5 x 10^5 cells/vial $446 +
M1860-57 小鼠星形胶质细胞-成鼠 5 x 10^5 cells/vial $489 +
M1900 小鼠小胶质细胞 1 x 10^6 cells/vial $608 +
M1900-57 小鼠小胶质细胞 1 x 10^6 cells/vial $714 +
M1920 小鼠巨噬细胞 1 x 10^6 cells/vial $372 +
M1920-10 小鼠巨噬细胞 1 x 10^7 cells/vial $1483 +
M1920-2 小鼠巨噬细胞 2 x 10^6 cells/vial $588 +
M1920-5 小鼠巨噬细胞 5 x 10^6 cells/vial $932 +
M1920-57 小鼠巨噬细胞 1 x 10^6 cells/vial $417 +
M1920-57-2 小鼠巨噬细胞 2 x 10^6 cells/vial $649 +
M1930 小鼠骨髓单核细胞 10 x 10^6 cells/vial $322 +
M1930-25 小鼠骨髓单核细胞 25 x 10^6 cells/vial $632 +
M1930-57 小鼠骨髓单核细胞 10 x 10^6 cells/vial $365 +
M2300-57 小鼠真皮成纤维细胞 5 x 10^5 cells/vial $477 +
M2530 小鼠淋巴成纤维细胞 5 x 10^5 cells/vial $446 +
M2530-57 小鼠淋巴成纤维细胞 5 x 10^5 cells/vial $477 +
M2540 小鼠淋巴单核细胞 10 x 10^6 cells/vial $322 +
M2540-57 小鼠淋巴单核细胞 10 x 10^6 cells/vial $365 +
M2670-57 小鼠唾腺成纤维细胞 5 x 10^5 cells/vial $477 +
M3040 小鼠肺泡巨噬细胞 1 x 10^6 cells/vial $358 +
M3040-57 小鼠肺泡巨噬细胞 1 x 10^6 cells/vial $413 +
M3200 小鼠肺泡上皮细胞 1 x 10^6 cells/vial $495 +
M3200-57 小鼠肺泡上皮细胞 1 x 10^6 cells/vial $550 +
M3300 小鼠肺成纤维细胞 5 x 10^5 cells/vial $446 +
M3300-57 小鼠肺成纤维细胞 5 x 10^5 cells/vial $477 +
M4100 小鼠肾小管上皮细胞 5 x 10^5 cells/vial $446 +
M4100-57 小鼠肾小管上皮细胞 5 x 10^5 cells/vial $489 +
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