Alizarin Red S (ARS), an anthraquinone dye, has been widely used to evaluate calcium deposits in cell culture. The ARS staining is quite versatile because the dye can be extracted from the stained monolayer of cells and readily assayed.
ScienCell’s ARS Staining Quantification Assay (ARed-Q) provides a sensitive tool for the recovery and semi-quantification of ARS in a stained monolayer of cells. Mineralization is assessed by extraction of calcified mineral at low pH, neutralization with ammonium hydroxide, and colorimetric detection at 405 nm in a 96-well format. This assay is more sensitive than the cetylpyridinium chloride (CPC) extraction method, improving the detection of weakly mineralizing monolayers [1]. It provides a wider linear range: destained ARS dye ranging from 30 µM to 4 mM shows a linear relationship with the absorbance at 405 nm, making dilutions of samples prior to measurement unnecessary.
We have applied this quantification assay to the osteogenesis induction of human mesenchymal stem cells (hMSCs). Cells were cultured in different osteogenic differentiation medium for 18 days, fixed for ARS staining and quantified for mineral deposit using the kit. There is significant difference between the untreated control, the induced samples, and the different induction conditions (Figure 2). ScienCell’s ARed-Q assay can be applied for mesenchymal stem cell osteogenic differentiation, tumor characterization, and osteognic compound screening.
货号 | 8678 |
产地 | 美国 |
缩写 | ARed-Q |
规格 | 100 Tests |
用途 | 科研 |
储存 | 室温 |
运输 | 胶冰 |
货号 | 产品名称 | 规格 | 价格 | 指令 | |
8258 | 碱性磷酸酶活力检测试剂盒 ALP | 500 tests | $380 | + | |
8268 | 线粒体分离试剂盒 MITOISO | 100 tests | $321 | + | |
8278 | 细胞色素C氧化酶检测试剂盒 COX | 100 tests | $347 | + | |
8278 | 细胞色素C氧化酶检测试剂盒 COX | 100 tests | $347 | + | |
8288 | 碱性磷酸酶染色试剂盒(红) ALPr | 500 tests | $432 | + | |
8298 | 人间充质干细胞脂肪形成检测试剂盒 HMSC-A PCR | 50 reactions | $311 | + | |
8308 | L-乳酸盐检测试剂盒 LAC | 100 tests | $141 | + | |
8318 | 柠檬酸合成酶检测试剂盒 CS | 50 tests in cuvette or 250 tests in 96-well plate | $458 | + | |
8318 | 柠檬酸合成酶检测试剂盒 CS | 50 tests in cuvette or 250 tests in 96-well plate | $458 | + | |
8328 | 人间充质干细胞软骨形成检测试剂盒 HMSC-C PCR | 50 reactions | $311 | + | |
8338 | 谷氨酸检测试剂盒 GLU | 100 tests | $399 | + | |
8358 | 谷氨酸脱氢酶检测试剂盒 GDH | 100 tests | $399 | + | |
8368 | NAD/NADH检测试剂盒 NAD | 100 tests | $401 | + | |
8368 | NAD/NADH检测试剂盒 NAD | 100 tests | $401 | + | |
8378 | 阿尔新蓝染色试剂盒 ABlue | 100 tests | $117 | + | |
8388 | 储液瓶 传统风格 125ml 45mm颈尺寸 带盖 灭菌 | 100 tests | $354 | + | |
8398 | 葡萄糖6磷酸酶检测试剂盒 G6P | 100 tests | $256 | + | |
8408 | 已糖激酶检测试剂盒 HK | 100 tests | $319 | + | |
8418 | 葡萄糖检测试剂盒 GL | 100 tests | $284 | + | |
8428 | 葡糖6磷酸脱氢酶检测试剂盒 G6PDH | 100 tests | $256 | + | |
8438 | 尿酸检测试剂盒 US | 100 tests | $374 | + | |
8448 | 铁检测试剂盒 | 100 tests | $305 | + | |
8458 | 黄嘌呤氧化酶检测试剂盒 XO | 100 tests | $138 | + | |
8458 | 黄嘌呤氧化酶检测试剂盒 XO | 100 tests | $138 | + | |
8468 | 甘油检测试剂盒 GLY | 100 tests | $222 | + | |
8478 | 丙氨酸转氨酶检测试剂盒 ALT | 100 tests | $297 | + | |
8498 | 甘油三酸酯检测试剂盒 TG | 100 tests | $285 | + | |
8508 | 3-甘油磷酸脱氢酶检测试剂盒 GPDH | 100 tests | $423 | + | |
8518 | 总谷胱甘肽检测试剂盒 TGA | 100 tests | $258 | + | |
8518 | 总谷胱甘肽检测试剂盒 TGA | 100 tests | $258 | + |