ScienCell's GeneQuery? human astrocyte cell biology qPCR array kit (GQH-AST) is designed to facilitate gene expression profiling of key genes involved in (i) human astrocyte development and maintenance; (ii) genes that are much up-regulated in astrocytes; and (iii) related signaling pathways, such as Notch, TGFB, JAK/STAT, and MAPK pathways. 88 genes are selected in this kit based on database and literature research.
GeneQuery? PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery? Human Astrocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AFF2 |
CD44 |
CRYAB |
EPM2A |
HEY1 |
ITGA3 |
LMO2 |
NHLRC1 |
POMC |
SCN1B |
TGFB2 |
β-actin |
|
B |
ATRX |
CDKL5 |
DLK1 |
FMR1 |
HOPX |
JAG1 |
LPPR4 |
NOTCH1 |
PRDM16 |
SLC25A18 |
TGFB3 |
GAPDH |
|
C |
BEND6 |
CDKN2B |
DNER |
FOXG1 |
HTT |
JAK1 |
MAPK10 |
NOTCH3 |
PRRX1 |
SMAD2 |
TGFBR3 |
LDHA |
|
D |
BHLHE40 |
CHRNA4 |
DPF3 |
FXR1 |
IGFBP7 |
KLF5 |
MAPK14 |
NR2E1 |
PTPRE |
SMAD3 |
TH |
NONO |
|
E |
BHLHE41 |
CLN8 |
DUSP1 |
FXR2 |
IL1RAPL1 |
LEP |
MECP2 |
NUFIP1 |
RBPJ |
STAT2 |
TM4SF1 |
PPIH |
|
F |
BMP7 |
CNTF |
DUSP18 |
GABRG2 |
IL2RA |
LGALS3 |
MVP |
NUFIP2 |
RPS27A |
STAT6 |
TSPAN7 |
GDC |
|
G |
CACNA1H |
CNTFR |
EFHC1 |
GFAP |
IL6 |
LHX2 |
NFIC |
OTX1 |
S100A6 |
SYNC |
UBE3A |
PPC |
|
H |
CASP3 |
COBL |
EMX2 |
GLIPR1 |
IRS2 |
LIF |
NFIX |
PHF11 |
SCN1A |
TDRD7 |
ZFP3 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK009 |
| 产地 | 北美 |
| 缩写 | GQH-AST |
| 规格 | 96Tests |
| 用途 | 科研 |
| 储存 | 4度 |
| 运输 | 胶冰 |
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