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人真皮成纤维细胞-成人 HDF-a

英文名:Human Dermal Fibroblasts-adult

货号:2320

价格:$248

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    成纤维细胞是来源于中胚层的一种间充质细胞。它被广泛应用于细胞和分子生物学的研究。这主要是因为它是一种较容易培养的,耐受性较好的细胞,可应用于基因转染、显微注射等多种操作。现已证明不同部位的成纤维细胞有着本质的不同,组织里的成纤维细胞处于一种动态的平衡之中,这种平衡影响着健康,以及已愈合软组织的结构完整性。成纤维细胞分泌一种非刚性细胞外基质,其中含有I型和III型胶原质。此外真皮纤维细胞在炎症刺激下还能分泌大量透明质酸。在创伤愈合过程中,真皮成纤维细胞从迁移、再生表型转变为可收缩、基质聚集表型。
    人真皮成纤维细胞(成人)提取于人皮肤组织,原代冻存。每管含有细胞数>5x10^5cells/ml,此细胞通过Fibronectin免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

推荐培养基:成纤维细胞培养基 (FM, Cat. #2301)
货号2320
产地美国
缩写HDF-a
规格5 x 10^5 /1ml
用途科研
储存液氮
运输干冰
1.) Rouabhia M, Park H, Meng S, Derbali H, Zhang Z. (2013) "Electrical Stimulation Promotes Wound Healing by Enhancing Dermal Fibroblast Activity and Promoting Myofibroblast Transdifferentiation." PLoS One. 8: e71660. 

2.) Ploeger DT, Hosper NA, Schipper M, Koerts JA, de Rond S, Bank RA. (2013) "Cell plasticity in wound healing: paracrine factors of M1/ M2 polarized macrophages influence the phenotypical state of dermal fibroblasts." Cell Commun Signal. 11: 29. doi: 10.1186/1478-811X-11-29.

3.) Huang SM, Cheung CW, Chang CS, Tang CH, Liu JF, Lin YH, Chen JH, Ko SH, Wong KL, Lu DY. (2011) "Phloroglucinol derivative MCPP induces cell apoptosis in human colon cancer." J Cell Biochem. 112: 643-52. 

4.) Kang TH, Guibinga GH, Jinnah HA, Friedmann T. (2011) "HPRT deficiency coordinately dysregulates canonical Wnt and presenilin-1 signaling: a neuro-developmental regulatory role for a housekeeping gene " PLoS One. 6: e16572. 

5.) Lee W, Debasitis JC, Lee VK, Lee JH, Fischer K, Edminster K, Park JK, Yoo SS. (2009) " Multi layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication. " Biomaterials. 30: 1587-95.

6.) Hao S, Baltimore D. (2009) "The stability of mRNA influences the temporal order of the induction of genes encoding inflammatory molecules." Nat Immunol. 10: 281-8.

7.) Lee W, Debasitis JC, Lee VK, Lee JH, Fischer K, Edminster K, Park JK, Yoo SS. (2009) "Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication." Biomaterials. 30: 1587-95.

8.) Montes CL, Chapoval AI, Nelson J, Orhue V, Zhang X, Schulze DH, Strome SE, Gastman BR. (2008) "Tumor-induced senescent T cells with suppressor function: a potential form of tumor immune evasion." Cancer Res. 68: 870-9. 
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