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人类HIF1信号响应qPCR检测试剂盒
Human HIF1 Signaling Response qPCR Array Kit
品牌:
ScienCell
货号:
GK036
价格:
$286
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ScienCell's GeneQuery? Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation.? HIF1 activity is a major component of the human response to hypoxia.? Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:

  • Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2
  • Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1
  • Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF
  • Metabolism: PKM, STC2, DHX35, DDX11, ERO1A

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery? Human HIF1 Signaling Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

?

?

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCB1

BHLHE40

COL18A1

DUSP6

HMOX1

LOXL2

NEK4

PKM

PRC1

RRAS

TSFM

β-actin

B

ADORA2A

BRCA1

COL1A1

ELK1

IGFBP3

MAP3K5

NOTCH4

PLCG2

PSME3

RRM2

TXNIP

GAPDH

C

ADORA2B

CCNE1

COL1A2

ERO1A

INHBA

MCM4

P4HA1

PLOD1

PTGS1

SKP2

UBE2S

LDHA

D

AK3

CCNE2

COL5A1

EXOSC2

INHBE

MEF2A

P4HA2

PLOD2

PTPRB

SMAD9

VEGFA

NONO

E

ANGPTL4

CCNF

CXCR4

FOS

INSR

MLLT10

PAM

POLE2

PTPRR

STC1

VEGFC

PPIH

F

BARD1

CDC6

DDX11

GADD45B

KDM3A

MMP2

PDZD2

POLR3K

RB1

STC2

VLDLR

GDC

G

BCL2

CDK1

DHFR

GINS2

LEPR

MXI1

PELI2

PPARD

RFC5

TFAM

ZNF292

PPC

H

BCL6

CDKN1C

DHX35

HES1

LOX

NDRG1

PGF

PPARG

RNH1

TRIP13

ZWINT

NTC

?

*gene selection may be updated based on new research and development


货号GK036
产地美国
缩写GQH-HFT
规格96tests
用途科研
储存4度
运输胶冰
No Publication available at this time
There are no product questions yet.
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