ScienCell's GeneQuery™ Human Cell Surface Markers qPCR Array Kit (GQH-CSM) surveys a panel of 88 cell surface markers. Cell surface markers are proteins attached to the cell membrane that may serve as markers for the identification and characterization of specific cell types.They have also been linked to transmembrane transport and inter-cellular communication. Brief examples of how genes may be grouped are shown below:

• B-cell surface markers: CD1C, CD28, CD79, CD80, CD86, CR1, FCGR2A, ITGA3, MS4A1, NT5E
• T-cell surface markers: CD4, CD8, CD3, CD6, CD40LG, CTLA4, FAS, TNFRSF4
• Natural killer (NK) cell surface markers: CD2, CD7, CD96, CD244, CD247, IL12RB1, KLRs, NCAM1
• Macrophage and monocyte surface markers: CD33, CD63, CD69, CD70, CD74, DPP4, IL1R2, ITGA1, TNFRSF8
• Platelet surface markers: ITGA2B, ITGB3, SELP
• Dendritic cell surface markers: CD1A, CD40, CD83, CD86, CD209, IL3RA, ITGAX
• Granulocyte surface markers: C5AR1, CEACAM8, FCER1A, FCER2, FCGR3A
• Endothelial cell surface markers: ENG, ICAM2, MCAM, NOS3, PECAM1, TEK, VCAM1, VWF
• Epithelial cell surface markers: CD1D, EPCAM, KRT8, KRT18
• Smooth muscle cell surface markers: MYH9, MYH10, MYOCD
• Fibroblast surface markers: ALCAM, COL1A1, COL1A2

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Cell Surface Markers qPCR Array Plate Layout *

(8 controls in Bold and Italic)

Note: all gene names follow their official symbols by HGNC

 * gene selection may be updated based on new research and development