ScienCell's GeneQuery™ Human Noncanonical Wnt Signaling Pathways qPCR Array Kit (GQH-WAP) is designed to facilitate gene expression profiling of 88 key genes involved in alternative Wnt pathways. This kit emphasizes genes involved in planar cell polarity signaling and calcium signal transduction. Brief examples of how included genes may be grouped according to function are shown below:
- Planar cell polarity: DAAM1, CELSR1, FAT1, FZD7, VANGL1
- Calcium: CASR, CALM1, FOSL1, FZD4, NFATC1
- Signaling modulator: LRP5, LRP6, SPRY1, EFNB, CASR
- Receptors: WNT2B, WNT4, WNT5A, WNT9A, WNT11
- Target genes: RHOA, RACGAP1, CREBBP, CFL1, FUZ
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Noncanonical Wnt Signaling Pathways qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ATN1 |
CAPN2 |
CREBBP |
DGKE |
FAT4 |
FZD6 |
ITPR3 |
PFN1 |
RACGAP1 |
SPRY1 |
WNT4 |
β-actin |
|
B |
AXIN1 |
CASR |
CREBZF |
DVL1 |
FLNA |
FZD7 |
LIMK1 |
PLCD1 |
RGS18 |
TLR4 |
WNT5A |
GAPDH |
|
C |
AXIN2 |
CDX2 |
CSNK1E |
DVL2 |
FOSL1 |
GSK3B |
LRP5 |
PLCE1 |
RGS3 |
VANGL1 |
WNT5B |
LDHA |
|
D |
CALM1 |
CELSR1 |
DAAM1 |
DVL3 |
FUZ |
HGS |
LRP6 |
PLCG1 |
RHOA |
VANGL2 |
WNT6 |
NONO |
|
E |
CAMK2A |
CELSR2 |
DCHS1 |
EFNB1 |
FZD2 |
INTU |
MAPK8 |
PRICKLE1 |
ROR1 |
WNT11 |
WNT7A |
PPIH |
|
F |
CAMK2B |
CELSR3 |
DCHS2 |
FAT1 |
FZD3 |
INVS |
NFATC1 |
PRICKLE2 |
ROR2 |
WNT16 |
WNT7B |
GDC |
|
G |
CAMK4 |
CFL1 |
DGKB |
FAT2 |
FZD4 |
ITPR1 |
NFATC2 |
PRKCD |
RYK |
WNT2B |
WNT9A |
PPC |
|
H |
CAPN1 |
CFL2 |
DGKD |
FAT3 |
FZD5 |
ITPR2 |
PCDH8 |
PTK7 |
SIAH2 |
WNT3A |
WNT9B |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK027 |
| 产地 | 美国 |
| 缩写 | GQH-WAP |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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