ScienCell's GeneQuery™ Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit (GQH-FGF) is designed to facilitate gene expression profiling of 88 key genes involved in Fibroblast Growth Factor (FGF) signaling pathways. FGFs are involved in a wide variety of biological functions such as embryonic development, organogenesis, angiogenesis, tissue regeneration and wound healing. Through their binding with FGF receptors (FGFRs), FGFs activate and regulate many downstream signaling pathways, such as MAPK, JNK and STAT/NF-κB. Brief examples of how included genes may be grouped according to their functions are shown below:     

• Secreted FGFs
• FGF1 superfamily: FGF1, FGF2
• FGF4 superfamily: FGF4, FGF5
• FGF7 superfamily: FGF3, FGF7, FGF10
• FGF8 superfamily: FGF17, FGF18
• FGF9 superfamily: FGF9, FGF16, FGF20
• Endocrine FGFs: FGF19, FGF23
• Intracellular FGFs: FGF13, FGF14
• FGF receptors: FGFR1, FGFR2, FGFR3, FGFR5
• FGF signaling inhibitors: IL17RD, SPRY2, SPRY4, PTPN1, DUSP1, DUSP6
• Activated downstream pathways
• MAPK pathway: FRS2, GAB1, SOS1, PTPN11, SHC1, RAS, BRAF, MAPK1, MAPK3, MAP2K1, MAP2K2
• PI3K/AKT pathway: AKT1, AKT2, AKT3, PI3Ks, FOXO1, FOXO3, BAD, BAX, CASP9
• JNK pathway: MAPK8, MAPK9, MAPK14, RAC1, ATF2, MAP2K4, MAP2K6
• STAT/NF-κB pathway: STAT1, STAT3, NFKB1, NFKB2, REL, MMP9

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Fibroblast Growth Factor Signaling Pathway qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development