ScienCell's GeneQuery™ Human Skin Wound Healing qPCR Array Kit (GQH-SWH) is designed to facilitate gene expression profiling of 88 key genes involved in the wound repair process. Wound healing is a complex process that includes many biological events, such as phagocytosis, angiogenesis, neocollagenesis, epithelialization, and collagen remodeling. It can be generally divided into 3 stages: 1) hemostasis and coagulation, 2) inflammation, and 3) proliferation and tissue remodeling. Brief examples of how included genes may be grouped according to their functions are shown below:     

• Wound healing stages
• Hemostasis and coagulation: IL1A, IL1B, IL6, TNF, FGF2, IGF1, TGFs, HIF1A
• Inflammation: CXCL8, VEGFs, ELANE, CTSG, PRTN3, PLAU, TGFs, PDGFs, TNF, IFNG
• Proliferation and tissue remodeling:  EGF, FGF7, IGF1, IFNG, CSK, RAC1, RHOA, CDC42
• Extracellular Matrix components: COL1A2, COL3A1, COL4A1, COL14A1
• Cellular adhesion: CDH1, ITGAs, ITGBs, MMPs, F3, FGA, TIMP1, ACTA2, ACTC1, TAGLN
• Cytokines and chemokines: CCL2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL4, IL6
• Growth factors: CSF2, CSF3, EGF, CTGF, FGFs, PDGFs, TGFs

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Skin Wound Healing qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 * gene selection may be updated based on new research and development