ScienCell's GeneQuery™ Human Hemostasis qPCR Array Kit (GQH-HEM) profiles 88 key genes involved in stopping bleeding. Upon wounding, hemostasis generally involves vasoconstriction to prevent blood flow to the afflicted area, forming a temporary plug to inhibit bleeding, and then coagulation or blood clotting. Examples of disorders in hemostasis are hemophilia, thrombosis, and embolisms. Brief examples of how included genes may be grouped according to their function in hemostasis are shown below:
- Platelet activation: F2R, FCER1G, FYN, PLA2G4A, PIK3CB
- Clotting: F8, PF4, PLAT, THBD, COL4A1
- Coagulation: A2M, F7, PLAUR, C5AR1, C7
- Hemophilia: F2, F3, VWF, IL10, F5
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Hemostasis qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
A2M |
APP |
F3 |
FYN |
IL3 |
JAK3 |
PFN1 |
PLAUR |
PSAP |
SOS1 |
TMSB4X |
β-actin |
|
B |
ACTN1 |
C5AR1 |
F5 |
GNAQ |
IL5 |
LAT |
PIK3CB |
PLCG2 |
PTK2 |
SPARC |
TUBA4A |
GAPDH |
|
C |
ACTN2 |
C6 |
F7 |
IGF1 |
IL6 |
LYN |
PIK3CD |
PLEK |
SELP |
SRGN |
VAV1 |
LDHA |
|
D |
ADRA2A |
C7 |
F8 |
IGF2 |
ITGA2B |
MPL |
PIK3R1 |
PLG |
SEPP1 |
TGFB1 |
VAV2 |
NONO |
|
E |
AKT2 |
CLU |
FCER1G |
IL10 |
ITGB3 |
MTCH1 |
PIKFYVE |
PRF1 |
SERPINA1 |
THBD |
VAV3 |
PPIH |
|
F |
AKT3 |
COL4A1 |
FIGF |
IL2 |
ITPR1 |
P2RY12 |
PLA2G4A |
PRKCA |
SERPINE1 |
THBS1 |
VCL |
GDC |
|
G |
APBB1IP |
F2 |
FLNA |
IL2RA |
ITPR2 |
PECAM1 |
PLAT |
PRKCE |
SFTPA1 |
TIMP1 |
VEGFA |
PPC |
|
H |
APOA1 |
F2R |
FN1 |
IL2RB |
JAK2 |
PF4 |
PLAU |
PRKCQ |
SOD1 |
TLN1 |
VWF |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK056 |
| 产地 | 美国 |
| 缩写 | GQH-HEM |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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