ScienCell's GeneQuery™ Human Keratocyte Biology qPCR Array (GQH-KER) profiles 88 key genes involved in corneal keratocyte function. Keratocytes are specialized fibroblasts that play roles in wound healing, maintaining corneal transparency, and corneal component synthesis. Keratocyte pathologies include keratoconus and limbal stem cell deficiency. Below are brief examples of how included genes may be grouped according to their function in keratocyte biology:
- Markers: MUC1, CD34, KERA, KRT19, LUM
- Wound Healing: MMP12, MRC1, NFKB1, COL3A1, CCL2
- Cell Cycling: MAPK1, MYCN, PTN, ETS1, CAPN9
- Oxidative Stress Response: RRAS, NOS2, NQO1, MAF, HMOX1
- Keratoconus Development: IGFBP3, MMP1, SSTR1, CRX, TNFAIP6
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Keratocyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACTA1 |
CCL2 |
COL1A1 |
CRYAA |
F13A1 |
GSTK1 |
KERA |
MAPK3 |
NCAM1 |
PTN |
TGFBR1 |
β-actin |
|
B |
ACTA2 |
CCL7 |
COL1A2 |
CRYAB |
FASLG |
HMOX1 |
KRT1 |
MMP1 |
NFKB1 |
RRAS |
TGFBR2 |
GAPDH |
|
C |
ALDH1A1 |
CD34 |
COL3A1 |
CRYBB1 |
FGF2 |
ICAM1 |
KRT12 |
MMP12 |
NFKB2 |
SDC1 |
TIMP1 |
LDHA |
|
D |
ATF1 |
CD68 |
COL5A1 |
CRYGD |
FGFR1 |
IGF1R |
KRT19 |
MMP2 |
NOS2 |
SOD2 |
TIMP2 |
NONO |
|
E |
BGN |
CDK1 |
COL5A2 |
CXADR |
FGFR2 |
IGFBP3 |
LCN2 |
MMP3 |
NQO1 |
SPARC |
TIMP3 |
PPIH |
|
F |
BMP4 |
CFL1 |
COL6A2 |
ECM1 |
FN1 |
IGFBP5 |
LUM |
MRC1 |
PCNA |
SSTR1 |
TKT |
GDC |
|
G |
C12orf57 |
CHST6 |
COL7A1 |
EPHX1 |
FOS |
IL1A |
MAF |
MUC1 |
PDGFRB |
TC2N |
TNFAIP6 |
PPC |
|
H |
CAPN9 |
CLU |
CRX |
ETS1 |
FOSL1 |
IL6 |
MAPK1 |
MYCN |
PTGDS |
TGFB1 |
VCL |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK074 |
| 产地 | 美国 |
| 缩写 | GQH-KER |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK001 | 人类细胞凋亡qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK002 | 人类多能干细胞qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK003 | 人类细胞周期qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK005 | 人上皮细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK006 | 人类神经发育和再生qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK007 | 人体神经可塑性qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK008 | 人体神经传递和膜转运qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK009 | 人类星形胶质细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK010 | 人类黑素细胞发育和色素沉着qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK011 | 人类黑素细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK013 | 人类星形胶质细胞转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK017 | 人血管生成qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK018 | 人内皮细胞表型异质性qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK019 | 人内皮细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK020 | 人B细胞受体信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK021 | 人类T细胞受体信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK022 | 人内吞作用qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK023 | 人内皮细胞分化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK024 | 人体剪切应力和机械传导qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK025 | 人类Notch信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK026 | 人类Wnt信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK027 | 人类非经典Wnt信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK028 | 人类Wnt信号靶基因qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK029 | Human Tie2 / Angpt信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK034 | 人肺纤维化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK035 | 人体缺氧反应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK036 | 人类HIF1信号响应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK037 | 人巨噬细胞细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK038 | 人类吞噬作用qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK039 | 人类先天免疫反应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
