ScienCell's GeneQuery™ Human Osteogenic Differentiation qPCR Array (GQH-OST) facilitates gene expression profiling of 88 key genes involved in the differentiation of mesenchymal stem cells into osteocytes, osteoblasts, and osteoclasts. Osteocytes are most commonly found in mature bone tissue and capable of signal transmission over long distances. Osteoblasts and osteoclasts are bone cells that synthesize and break down bone, respectively. Brief examples of how included genes may be grouped according to their function are shown below:
- Osteocyte marker: BGN, FGF23, PDPN, HYOU1, SOST
- Osteoblast marker: GNL3, CD44, OMD, MME, SCUBE3
- Osteoclast marker: CA2, CALCR, CTSK, MMP9, TNFRSF11A
- RUNX2 suppressor: GLI3, HES1, STAT1, TWIST1, HAND2
- BMP signaling: BMP2, BMP4, SMAD4, SMAD5, SMAD9
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Osteogenic Differentiation qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ALCAM |
CA2 |
CSNK2A1 |
FGF2 |
FZD9 |
HYOU1 |
MAF |
NKX3-2 |
RBPJ |
SMO |
TCF7 |
β-actin |
|
B |
ALPL |
CALCR |
CTNNB1 |
FGF23 |
GLI2 |
IBSP |
MCAM |
NOTCH1 |
RUNX2 |
SOST |
TGFB1 |
GAPDH |
|
C |
ATF4 |
CAPG |
CTSK |
FGFR1 |
GLI3 |
IGF1R |
MEPE |
NOTCH2 |
SATB2 |
SOX9 |
TNFRSF11A |
LDHA |
|
D |
BCAP31 |
CD44 |
DCN |
FGFR2 |
GNL3 |
IGFBP3 |
MME |
OMD |
SCUBE3 |
SP7 |
TNFSF11 |
NONO |
|
E |
BGN |
CD59 |
DMP1 |
FGFR3 |
HAND2 |
ITGB1 |
MMP14 |
PCOLCE |
SMAD1 |
SPARC |
TPO |
PPIH |
|
F |
BMP2 |
COL1A1 |
DSTN |
FN1 |
HES1 |
LEF1 |
MMP9 |
PDPN |
SMAD4 |
SPP1 |
TWIST1 |
GDC |
|
G |
BMP4 |
COL1A2 |
ENG |
FOSB |
HEY1 |
LRP5 |
MSX2 |
PHEX |
SMAD5 |
STAT1 |
TXLNG |
PPC |
|
H |
BMPR1A |
COL2A1 |
FGF18 |
FOSL1 |
HIVEP3 |
LRP6 |
NFATC1 |
PRKCD |
SMAD9 |
TAZ |
WNT5A |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK080 |
| 产地 | 美国 |
| 缩写 | GQH-OST |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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