ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance. This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring. Brief examples of how included genes may be categorized are shown below:
- Gap junctions: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1
- Adherens junctions: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2
- Tight junctions: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)
- Desmosomes: DSC1, DSG1, DSP, ITGB4, JUP
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Cell Junctions qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACTA1 |
CADM1 |
CDH19 |
CELSR3 |
CLDN8 |
CXADR |
DSP |
GJA5 |
JAM3 |
NECTIN3 |
PECAM1 |
β-actin |
|
B |
ACTA2 |
CADM3 |
CDH2 |
CGN |
CLTA |
DCHS1 |
F11R |
GJB1 |
JUP |
OCLN |
PVR |
GAPDH |
|
C |
ACTB |
CDH1 |
CDH3 |
CLDN1 |
CLTB |
DCHS2 |
FAT1 |
GJB6 |
LEF1 |
PAK1 |
RHOA |
LDHA |
|
D |
ACTC1 |
CDH10 |
CDH5 |
CLDN10 |
CLTC |
DNM1 |
FAT2 |
ICAM1 |
MAGI1 |
PANX1 |
SNAI1 |
NONO |
|
E |
ACTG1 |
CDH11 |
CDX1 |
CLDN2 |
CLTCL1 |
DSC1 |
FAT3 |
ILK |
MAGI2 |
PARD3 |
SP1 |
PPIH |
|
F |
ACTG2 |
CDH13 |
CDX2 |
CLDN4 |
CSNK1D |
DSC2 |
FAT4 |
ITGB1 |
MYLK |
PARD6A |
SRC |
GDC |
|
G |
ANG |
CDH15 |
CELSR1 |
CLDN5 |
CTNNB1 |
DSG1 |
GJA1 |
ITGB4 |
NECTIN1 |
PCDH7 |
TJP1 |
PPC |
|
H |
AP2M1 |
CDH16 |
CELSR2 |
CLDN7 |
CTNND1 |
DSG3 |
GJA4 |
JAM2 |
NECTIN2 |
PCDH8 |
WNK4 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK090 |
| 产地 | 美国 |
| 缩写 | GQH-JXN |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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