ScienCell's GeneQuery™ Human Schwann Cell Biology qPCR Array Kit (GQH-SCH) is designed to facilitate gene expression profiling of 88 key genes involved in human Schwann cell biology, such as peripheral nerve formation and regeneration, Schwann cell development and differentiation, myelination and extracellular matrix (ECM) synthesis. Genes implicated in selected Schwann cell related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

• Schwann cell markers: SOX10, NGFR, SOX2, GAP43, NCAM1, EGR2, POU3F1, MBP, MPZ, S100B, PMP22, GFAP

• Schwann cell myelination: MOG, CNP, SOX10, EGR2, NFATC4, NRG1, CD9, HEXB, IFNG, NTF3, PLP1

• Maturation and differentiation: WASL, NCAM1, VGF, IL6, BDNF, STAT1, PTN, CD40, LIF, MEF2C
• Schwann cell plasticity and dedifferentiation: ERBB2, RAF1, MAPK1, MAPK3, MAPK11, MAPK14, JUN, ARTN, BDNF, GDNF, SOX2, GJA1
• Nerve regeneration support: NTN1, UNC5B, SHH, GAP43, CDH1, TYRP1, FABP7, SOSTDC1, KCNJ10, LRRTM4, OLIG1
• ECM synthesis in the peripheral nerve trunk: laminins, collagens, FN1, ICAM1, PLAT, PLAUR, NRG1, ITGB1, ERBB2, ERBB3
• Genes implicated in
• hereditary motor and sensory neuropathies: WNK1, KIF1A, FAM134B, SCN9A, APOB, IKBKAP, IFNB1, HLAs, AQP4, PLP1, IFNG
• schwannoma: NF1, NF2, SMARCB1, KIT, PRKAR1A, GFAP, EZR, SOX10, NGFR, MBP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Schwann Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development