Preadipocytes are fibroblast-like precursor cells that can proliferate and differentiate into mature adipocytes. Preadipocytes play an important role in metabolic homeostasis and nutrient signaling. ScienCell's GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Kit (GQH-PAD) is designed to facilitate gene expression profiling of 88 key genes involved in human preadipocyte cell biology, such as cell proliferation, differentiation, ECM formation and lipid metabolism. Genes implicated in obesity and liposarcoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

• Preadipocyte cell markers: ADIPOQ, LEP, HOXC8/9, TBX1, TMEM26, UCP1, CIDEA, PRDM16, SLC7A10, P2RX5
• Proliferation: CCND1, PROKR1, LYRM1, FTO, SLC2A4, PDGFA, IL4, IGF1, FGF5, MAPK1/3, TNFSF10
• Differentiation and adipogenesis: PPARG, CEBPA/B, KLF2/5/15, SOCS1/3, INSR, DLK1, ADD1, SREBF1
• ECM and cytoskeleton: MMP1/2/3, FAP, DPP4, TGFB1, COL1A1/2, SMAD2/4
• Lipid metabolism: FABP5/7, LBP, LPL, PLIN2, ACSL1, LIPC
• Obesity: MC4R, UCP3, ADRB2/3, POMC, FFAR4, CARTPT
• Liposarcoma: FUS, MDM2, HMGA2, DDIT3, CDK4, EWSR1, DES, VIM

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Plate Layout *

(8 controls in Bold and Italic)

Note: all gene names follow their official symbols by HGNC

* gene selection may be updated based on new research and development