ScienCell's GeneQuery™ Human Neural Differentiation Markers qPCR Array Kit (GQH-NDM) is designed to facilitate gene expression profiling of 40 marker genes involved in neural differentiation to specific neural lineages. Brief examples of how included genes may be grouped according to their functions are shown below:
- Immature neuron markers: DCX, NEUROD1, STMN1, TUBB3
- Mature neuron markers: MAP2, NEFH, NEFM, RBFOX3
- Astrocyte markers: GFAP, GLUL, S100B, SLC1A2, S100A6, NFIX, NFIC
- Schwann cell markers: GAP43, MPZ, NCAM1, S100A1, NGFR, EGR2, POU3F1
- Oligodendrocyte markers: CSPG4, MBP, MOG, OLIG1, OLIG2, PDGFRA, SOX10
- Radial glial cell markers: CDH2, FABP7, GFAP, HES1, HES5, NES, PAX6, VIM
- Neuroepithelial cell markers: CDH1, HES1, NES, NOTCH1, OCLN, SOX2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Neural Differentiation Markers qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
CDH1 |
GLUL |
NEFH |
OCLN |
S100A6 |
β-actin |
CDH1 |
GLUL |
NEFH |
OCLN |
S100A6 |
β-actin |
|
B |
CDH2 |
HES1 |
NEFM |
OLIG1 |
S100B |
GAPDH |
CDH2 |
HES1 |
NEFM |
OLIG1 |
S100B |
GAPDH |
|
C |
CSPG4 |
HES5 |
NES |
OLIG2 |
SLC1A2 |
LDHA |
CSPG4 |
HES5 |
NES |
OLIG2 |
SLC1A2 |
LDHA |
|
D |
DCX |
MAP2 |
NEUROD1 |
PAX6 |
SOX10 |
NONO |
DCX |
MAP2 |
NEUROD1 |
PAX6 |
SOX10 |
NONO |
|
E |
EGR2 |
MBP |
NFIC |
PDGFRA |
SOX2 |
PPIH |
EGR2 |
MBP |
NFIC |
PDGFRA |
SOX2 |
PPIH |
|
F |
FABP7 |
MOG |
NFIX |
POU3F1 |
STMN1 |
GDC |
FABP7 |
MOG |
NFIX |
POU3F1 |
STMN1 |
GDC |
|
G |
GAP43 |
MPZ |
NGFR |
RBFOX3 |
TUBB3 |
PPC |
GAP43 |
MPZ |
NGFR |
RBFOX3 |
TUBB3 |
PPC |
|
H |
GFAP |
NCAM1 |
NOTCH1 |
S100A1 |
VIM |
NTC |
GFAP |
NCAM1 |
NOTCH1 |
S100A1 |
VIM |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK112 |
| 产地 | 美国 |
| 缩写 | GQH-NDM |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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