ScienCell's GeneQuery™ Human Thymocyte Development Markers qPCR Array Kit (GQH-TDM) is designed to facilitate gene expression profiling of 40 marker genes involved in thymopoiesis.  The tightly regulated lineages of common lymphoid progenitors (CLPs) consist of several maturational stages based on the expression of cell surface markers, including double negative stage (CD4- and CD8-), double positive stage (CD4+ and CD8+), and single positive stage (CD4+ or CD8+). The CD4+ T cells can further differentiate into a number of effectors subsets such as Th1, Th2, Th9, Th17, and Tfh. Brief examples of how included genes may be grouped according to their functions are shown below:

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

• CLP markers: MME, CD34, PTPRC, IL7R, FLT3
• Double negative thymocyte markers: CD1A, CD3D, CD3E, CD3G, CD5, CD7, CD200, IL2RA
• Double positive thymocyte markers: CD1A, CD4, CD5, CD7, CD8A, CD8B, CD38, SELL, CD200
• CD4+ T cell markers: CD3D, CD3E, CD3G, CD4, PTPRC, IL2RA, SELL
• CD8+ T cell markers: CD3D, CD3E, CD3G, CD8A, CD8B, GZMB
• Regulatory T cell markers: IL2RA, ENTPD1, NT5E, ITGAE, FOXP3
• Markers of T cell effector subsets
• Th1: FASLG, CXCR3, CCR5, IL12RB1, IL18R1
• Th2: CXCR3, CCR3, CCR4, CCR8, PTGDR2
• Th9: CCR6, IL9
• Th17: PTPRC, IL6R, KLRB1, CCR4, CCR6, IL13RA1, IL21R
• Tfh: CXCR5, PDCD1                                              

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Thymocyte Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note: all gene names follow their official symbols by HGNC

* gene selection may be updated based on new research and development