ScienCell's GeneQuery™ Human Innate Lymphoid Cell Development Markers qPCR Array Kit (GQH-ILC) is designed to facilitate gene expression profiling of 24 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into innate lymphoid cells (ILCs), natural killer (NK) cells, and lymphoid tissue inducer (LTi) cells. Brief examples of how included genes may be grouped according to their functions are shown below:

• CLP markers: MME, CD34, IL7R, FLT3
• Immature NK cell markers: CD33, PROM1, CD34, KIT, IL2RB, KLRK1
• Mature NK cell markers: FCGR3A, IL2RA, NCAM1, B3GAT1, KLRD1, KLRC1, NCR1/2/3
• ILC progenitor markers: CD34, KIT, IL7R
• ILC markers: NCAM1, KLRD1, ITGAE, IL7R, KLRB1, PTPRC, NCR1, RORC
• LTi cell markers: CD4, IL7R, CCR6

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Innate Lymphoid Cell Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note: all gene names follow their official symbols by HGNC

* gene selection may be updated based on new research and development