Bone Marrow Mononuclear Cells (BMMC) are located in the bone marrow and have regenerative capabilities. Primary BMMC are a heterogeneous population of single nucleus cells which include hematopoietic stem cells, hematopoietic progenitor cells, immature monocytes, and immature lymphocytes. Recent studies suggest that BMMC are suitable as a cell therapy to treat a variety of diseases such as cardiovascular disease, type 1 diabetes, and ischemic stroke. BMMC may also be used for bone tissue engineering. Primary mouse BMMC (MBMMC) can be utilized to further study BMMC as a potential cell therapy. MBMMC from ScienCell Research Laboratories are isolated from healthy adult C57BL/6 mouse bone marrow. MBMMC are depleted of erythrocytes and bone marrow macrophages, cryopreserved immediately after isolation, and delivered frozen. MBMMC are a mixed population of cells that include hematopoietic stem cells and progenitor cells, monocytes, and lymphocytes. Each vial contains at least 10 million cells in 1 ml volume. MBMMC are quality control tested for viability. MBMMC are negative for mycoplasma, bacteria, yeast, and fungi. MBMMC can be maintained for a short period of time in culture using the conditions provided by ScienCell Research Laboratories. MBMMC are not intended for long-term culture and should be used promptly for experiments. Recommended Medium It is recommended to use HematoGro Medium (HemGM, Cat. #5501) for short-term maintenance of MBMMC in vitro.