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Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.
MDF from ScienCell Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse skin. MDF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MDF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. MDF are negative for mycoplasma, bacteria, yeast, and fungi. MDF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MDF in vitro.
货号 | M2300-57 |
产地 | 北美 |
缩写 | MDF |
规格 | 5 x 10^5 cells/vial |
用途 | 科研 |
储存 | 液氮 |
运输 | 干冰 |
小鼠细胞系统
小鼠细胞系统
货号 | 产品名称 | 规格 | 价格 | 指令 | |
M1830 | 小鼠脊髓星形胶质细胞 | 5 x 10^5 cells/vial | $446 | + | |
M1830-57 | 小鼠脊髓星形胶质细胞 | 5 x 10^5 cells/vial | $489 | + | |
M1840 | 小鼠脑干星形胶质细胞 | 5 x 10^5 cells/vial | $446 | + | |
M1850 | 小鼠中脑星形胶质细胞 | 5 x 10^5 cells/vial | $446 | + | |
M1860-57 | 小鼠星形胶质细胞-成鼠 | 5 x 10^5 cells/vial | $489 | + | |
M1900 | 小鼠小胶质细胞 | 1 x 10^6 cells/vial | $608 | + | |
M1900-57 | 小鼠小胶质细胞 | 1 x 10^6 cells/vial | $714 | + | |
M1920 | 小鼠巨噬细胞 | 1 x 10^6 cells/vial | $372 | + | |
M1920-10 | 小鼠巨噬细胞 | 1 x 10^7 cells/vial | $1483 | + | |
M1920-2 | 小鼠巨噬细胞 | 2 x 10^6 cells/vial | $588 | + | |
M1920-5 | 小鼠巨噬细胞 | 5 x 10^6 cells/vial | $932 | + | |
M1920-57 | 小鼠巨噬细胞 | 1 x 10^6 cells/vial | $417 | + | |
M1920-57-2 | 小鼠巨噬细胞 | 2 x 10^6 cells/vial | $649 | + | |
M1930 | 小鼠骨髓单核细胞 | 10 x 10^6 cells/vial | $322 | + | |
M1930-25 | 小鼠骨髓单核细胞 | 25 x 10^6 cells/vial | $632 | + | |
M1930-57 | 小鼠骨髓单核细胞 | 10 x 10^6 cells/vial | $365 | + | |
M2300-57 | 小鼠真皮成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M2530 | 小鼠淋巴成纤维细胞 | 5 x 10^5 cells/vial | $446 | + | |
M2530-57 | 小鼠淋巴成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M2540 | 小鼠淋巴单核细胞 | 10 x 10^6 cells/vial | $322 | + | |
M2540-57 | 小鼠淋巴单核细胞 | 10 x 10^6 cells/vial | $365 | + | |
M2670-57 | 小鼠唾腺成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M3040 | 小鼠肺泡巨噬细胞 | 1 x 10^6 cells/vial | $358 | + | |
M3040-57 | 小鼠肺泡巨噬细胞 | 1 x 10^6 cells/vial | $413 | + | |
M3200 | 小鼠肺泡上皮细胞 | 1 x 10^6 cells/vial | $495 | + | |
M3200-57 | 小鼠肺泡上皮细胞 | 1 x 10^6 cells/vial | $550 | + | |
M3300 | 小鼠肺成纤维细胞 | 5 x 10^5 cells/vial | $446 | + | |
M3300-57 | 小鼠肺成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M4100 | 小鼠肾小管上皮细胞 | 5 x 10^5 cells/vial | $446 | + | |
M4100-57 | 小鼠肾小管上皮细胞 | 5 x 10^5 cells/vial | $489 | + |