The retina is a multi-layered structure lining the posterior segment of the eye. It consists of photoreceptors and retinal pigment epithelial cells (RPEpiC). RPEpiC are situated between the neurosensory retina and the choroid, forming the outer blood-retina barrier that controls the chemical composition of the subretinal space. RPEpiC have specific polarized protein distributions within the cell. In contrast to other epithelia which normally faces an apical lumen devoid of matrix, the RPEpiC apical plasma membrane is in direct contact with the extracellular matrix. RPEpiC play a critical role in regulating the adjacent photoreceptor membrane turnover, retinoid metabolism, and protecting the retina from light-induced damages by the intracellular melanin, which suggests that RPEpiC may contribute to the pathogenesis of various ocular diseases such as proliferative retinopathy and age-related macular degeneration. HRPEpiC from ScienCell Research Laboratories are isolated from human retina. HRPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HRPEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HRPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HRPEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for culturing HRPEpiC in vitro.