Trabecular meshwork is located around the circumference of the root of the iris and has a slit opening into the Schlemm’s canal that drains aqueous to venous vessels. Trabecular meshwork cells (TMC) play an active role in the aqueous humor outflow and this pathway is intraocular pressuredependent. Injury or death of TMC, therefore, has been associated with the pathogenesis of openangle glaucoma. TMC further express receptors for neurotransmitters and neuropeptides, and respond to very low concentrations of a variety of vasoactive peptides and growth factors, allowing these cells to regulate the permeability of trabecular meshwork at multiple levels. TMC cultures provides an invaluable tool to study the functional control of trabecular meshwork, encouraging the development of more efficient strategies to treat glaucoma. HTMC from ScienCell Research Laboratories are isolated from the juxtacanalicular and corneoscleral regions of human eye. HTMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HTMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin and/or fibronectin. HTMC are negative for HIV1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HTMC are guaranteed to further expand for 15 population doublings under the conditions specified by ScienCell Research Laboratories. Recommended Medium It is recommended to use Trabecular Meshwork Cell Medium (TMCM, Cat. #6591) for culturing HTMC in vitro.